On the day Wales announced their 34-man Six Nations squad, Louis Rees-Zammit sensationally quit rugby to pursue an American football career.
Rees-Zammit’s departure delayed the announcement of the squad until 12:00 GMT.
Five uncapped players were selected by Warren Gatland, including Exeter lock Dafydd Jenkins, 21.
On Saturday, 3 February, Wales will open their campaign against Scotland at Principality Stadium, Cardiff.
New faces include Cardiff quartet Cameron Winnett, Alex Mann, Mackenzie Martin and Evan Lloyd, as well as Bath prop Archie Griffin.
The Cardiff-born Exeter wing Immanuel Feyi-Waboso, who represented Wales at age-grade level, is not included in the squad.
Feyi-Waboso told Wales he would not be considered for the 2024 Six Nations squad, and he could be named in the England party on Wednesday.
A calf injury will keep Cardiff number eight Taulupe Faletau out of the tournament. The 33-year-old hasn’t played since breaking his arm for Wales against Georgia in the World Cup in October 2023.
Tomas Francis, Henry Thomas, and Dillon Lewis, who all play club rugby outside Wales, have no place in the World Cup squad.
Francis has not retired from international rugby, but Gatland explained they had a conversation about Francis missing this tournament.
A number of players have been recalled by Gatland, including Scarlets fly-half Ioan Lloyd, Cardiff back row James Botham, and Ospreys centre Owen Watkin.